Practical guide

Immunohistochemistry is a technique that aims to localize a protein in cells within a tissue section by using a specific antibody, which will bind to an antigen of the biological tissue. This technique is widely used, for example, to identify and characterize a tumor (malignancy, classification...).

IHC protocol

BAP1 protein or BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) is a de-ubiquitination enzyme encoded by BAP1 gene located on human chromosome 3. BAP1 (BRCA1-associated protein 1) binds to the N-terminal domain of BRCA1 and is a tumor suppressor gene.

Solitary fibrous tumors (SFT) are uncommon fibroblastic neoplasms. Often diagnosed by their histological characteristics and CD34 ...

Discover our new mouse-specific rat monoclonal antibodies for IHC-P.

Find all the cross-reactions of our BioSB antibodies for use in research or in veterinary clinics.

Uroplakins Ia, Ib, II and III are structural proteins of urothelial cells differentiated. In non-neoplastic urothelial cells, Uroplakins are expressed in the luminal membrane of surface cells. 

A new marker for gastrointestinal cancers diagnosis.

Cadherin 17 is a non-conventional cadherins and is also referred to as LI-cadherin or CDH17. It is involved in tumor invasion and metastasis...

SOX-10 is a very sensitive melanomas marker (97% versus 91% for S-100). It is particularly sensitive for desmoplastic melanomas which are often difficult to detect by other markers of melanocytes. SOX-10 is moderately to strongly expressed in desmoplastic melanomas ...

Distinction of hepatic metastases from hepatocellular carcinomas (HCC) can present some difficulties. Arginase-1 is a marker recently described in the literature as a new highly specific and sensitive polyclonal antibody for HCC detection...

9-Core ALK-1 Cell Line and 3-Core Lung Cancer Cell Line Microarray consist of 2 mm diameter deposits of cell lines fixed with formalin and included in paraffin...

MDM2 is an important negative regulator of the p53 tumor suppressor. MDM2 is an inhibitor of the P53 transcription, and function like an E3 ubiquitin ligase recognizing the N-terminal domain of trans-activation (TAD) of the tumor suppressor p53. Already known for its oncogene role, it has been shown that MDM2...

SATB2 (Special AT-sequence rich-Binding Protein 2) is a new very specific marker for colorectal cancer (CRC). This protein is selectively expressed in glandular cells of the lower gastrointestinal tract and its expression is conserved in a large majority of primary and metastatic CRCs...

NKX3.1 has been identified as a marker for metastatic tumors. NKX3.1 is positive for prostate staining cells with nuclear staining. Most cases of urothelial carcinoma were found negative for NKX3.1, allowing to distinguish high-grade prostate adenocarcinomas and high-grade infiltrated urothelial carcinomas...

Pan Plus Cytokeratin is an excellent alternative to Pan Cytokeratin clone KL-1. This cocktail allows the detection of acid cytokeratins 10, 15, 16 and 19 

This antibody recognizes neoplastic and normal cells of epithelial origin but does not recognize cells from the epidermal basal layers.

Its specificity is to recognize cytokeratins 1, 2, 5, 6, 7, 8, 10, 11, 14, 16, 17, 18 and 19.

PAX-8 belongs to the “paired box” transcription factors protein family. This nuclear protein is involved in follicular cells development in the thyroid gland and in the expression of specific thyroid genes. Mutations in PAX-8 gene have been associated with dysgenesis, follicular carcinoma and atypical adenomas of the thyroid gland...

Galectin-3 is a 31 kDa lectin that binds to beta-galactosidase. Galectin-3 is normally expressed in the epithelia of many organs and various inflammatory cells, including macrophages, dendritic cells and Kupffer cells.

This antibody cocktail is intended for human P504S (also known as AMACR or α-Methylacyl-CoA-Racemase) and human p63 detection in formalin-fixed paraffin-embedded tissues.

Proteins of the High Mobility Group (HMG) are transcription factors expressed during embryonic development and are absent in normal adult tissues. This family is composed of three proteins: HMGI, HMGA2 (also known as HMGI-C) and HMGI (Y)...

The WesternBright ChemiPen allows you to write or draw on transfer membranes and reveal protein standards, for example.

Find here some scientific information relating the antibodies to be used for specific tissues in diagnosis.

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La microglie est une population de macrophages située dans le système nerveux central (SNC), dont le premier rôle est la défense immunitaire du SNC. Des études ont montré que ces cellules pouvaient être impliquées dans le développement de la maladie d’Alzheimer.

Le nouveau coronavirus (SARS-CoV-2) a été découvert début janvier dans la ville de Wuhan (Chine) et s'est propagé dans le monde entier en quelques mois. La recherche de traitement efficace passe par l'étude du virus et DiagOmics se mobilise aux côtés de la communauté scientifique pour le combattre.

Find here our range of antibodies and reagents for flow cytometry.

Find here all our antibodies directed against collagen fibers.

Our new compartmentalized boxes for staining your Western Blot gels or membranes are finally available !!!

HRP sensitive substrate for Western blots

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FLASHBLOT buffer improves protein transfer on western blot membranes..

Spectra Dye Antibody Labeling Kits contain everything you need to couple antibodies ...

Advansta Western Blot Strip-It buffer uniformly removes antibodies from Western blot membranes ...

The BioSB TintoRetriever pressure cooker rigorously controls the temperature and duration of antigenic or nucleic acid pre-treatment of tissues fixed with formalin and included in paraffin ...