Carcinomas Immunophenotyping using IHC

Carcinomas Group 1

CARCINOMAS

CK Cocktail CK7 CK20 CK, LMW CK, HMW CK5 p63 Vimentin TTF-1 Thyro
globulin
GCDFP-15 ER/PR CEA
(poly)
CDX-2 Villin Hep
Par1
RCC CD10 Beta
Catenin
A-1
Antitrypsin
Hepatocellular Carcinoma

+

+

+

+

+/-

Renal Cell Carcinoma

+

+

+

+

+

+

Bladder Adenocarcinoma + + +/- + +   + +   +
Salivary Gland Carcinoma + + + + + +   +  
Thyroid Carcinoma + + +   + + +  
Breast Carcinoma + + + + + + +/- +
Lung Adenocarcinoma + + + + + +  
Colorectal Adenocarcinoma + + + + + + + +
Prostatic Adenocarcinoma + + +
Transitional Cell Carcinoma + + + + + + + +
Ovarian Carcinoma, Non Mucinous + + + + + +
Cervical Carcinoma + +   +  
Sweat Gland Carcinoma + +     + +   +  
Pancreatic Carcinoma + + + +/- + +/-
Gastric Carcinoma + +     + + +  
Squamous Cell Carcinoma + + + + +
Endometrial Adenocarcinoma + + +       + +   +
Carcinomas Group 2
CARCINOMAS CK7 CK20 CDX-2 Villin TTF-1 Napsin A PSA/PSAP Caveolin-1 Mammaglobin GCDFP-15
Breast Carcinoma + + + +  
Lung Adenocarcinoma + + +  
Transitional Cell (Bladder) Carcinoma + +  
Colorectal Adenocarcinoma + + + +  
Prostatic Adenocarcinoma + +/-  
Squamous vs Transitional Carcinomas
CARCINOMAS CK,34bE12 p63 CK5 Thrombomodulin CK7 CK20 Uroplakin III COX-2
Squamous Carcinoma + + + +
Transitional Cell Carcinoma + + -/+ + + + + +
Small Cell vs Merkel Cell Carcinomas
CARCINOMAS TTF-1 CEA CK20 Chromogranin A E-Cadherin (nuclear) Neurofilament CD117 Synaptophysin
Merkel Cell Carcinoma + + + + + +
Small Cell Carcinoma + +/- +
Cutaneous Neoplasms
CARCINOMAS CD10 Androgen Receptor CK20 CD34 Ber-EP4 bcl-2 CK19
Basal Cell Carcinoma + + + + +
Trichoepithelioma + + + + +
Merkel Cell Carcinoma + + + +
Microcystic Adnexal Carcinoma +/- -/+ +  
Sebaceous Carcinoma +/- + + +/-
Sebaceous Adenoma + + +
Carcinomas: Differential Diagnosis
CARCINOMAS Androgen Receptor BCA-225 GDDFP-15 ER/PR Mammaglobin Ny-BR-1 PSA/PSAP CD44
Salivary Duct Carcinoma + + +
Breast Carcinoma +(aprocrine) + + +/- + + +
Prostate Carcinoma (nuclear) + + +
Prostate Lesions
CARCINOMAS PSA/PSAP P504s CK, 34bE12 p63 CK7 Thrombomodulin Uroplakin III PAX-2
Prostate Carcinoma + +
Urothelial Carcinoma + + + + +
Nephrogenic Adenoma + +/- + +
Lung Carcinomas
CARCINOMAS CD56 Chromogranin A Synaptophysin CK7 Napsin A p63 TTF-1 BG8 GLUT-1
Small Cell Carcinoma + -/+ +/- + +
Adenocarcinoma + + + + +/-
Squamous Cell Carcinoma + + +
Large Cell Neuroendocrine Carcinoma + + + +/- +/-    
Colon vs Ovarian Carcinoma
CARCINOMAS CK7 CK20 CEA CDX-2 Villin CA19-9 Ep-CAM WT1 CA-125 CK5/6 PAX-8
Ovarian Carcinoma, Serous + + + + + + + +
Ovarian Carcinoma, Mucinous +/- +/- + + + +  
Ovarian Endometrioid Carcinoma +   +/- + -/+ + +
Colon Carcinoma + + + + + +
Mucin Expression in Neoplasms
CARCINOMAS MUC1 MUC2 MUC5AC MUC6
Pancreatic Adenocarcinoma + +
Lung Carcinoma, Signet Ring + +  
Ovarian Adenocarcinoma + +  
Gastric Carcinoma -/+ + -/+
Cervical Adenocarcinoma + +
Esophageal Carcinoma + +  
Paget’s Extramammary + -/+ +
Cholangiocarcinoma + +/-
Salivary Duct Adenocarcinoma + +
Colon Carcinoma, Signet Ring +
Prostate Carcinoma +/-
Pancreatic Intraductal Papillary Carcinoma + + +
HCC
Adrenocortical Carcinoma
Breast Carcinoma +
Lung Carcinoma +  
Kidney Carcinoma +  
Bladder Carcinoma +  
Endometrial Carcinoma +
Ovarian Carcinoma, Mucinous + +  
Renal Cell Carcinoma +  
Urothelial Carcinoma +  
Appendiceal Adenocarcinoma + +
Barrett’s Esophagus + + +
Pancreatic Mucinous Cystic +
Breast Colloid Carcinoma + + +
Mucin Expression in Organs
CARCINOMAS MUC1 MUC2 MUC4 MUC5AC MUC6
Stomach + + + +
Small Intestine + +
Colon +  
Pancreas + +
Ampullary Carcinoma (Enteric vs Ductal)
CARCINOMAS CK17 Hep-Par1 CDX-2
Enteric +
Ductal + -/+
Colon vs Prostate Adenocarcinoma
CARCINOMAS CDX-2 CK20 CEA CA19-9 PSA P504s
Colon Adenocarcinoma + + + +
Prostate Adenocarcinoma + +